Practical questions
During the GCSE Biology course you will complete practical activities from eight Practical Activity Groups (PAGs).
The exams will include questions about some of these experiments. You may also be asked to apply what you know to unfamiliar practical contexts, which will draw on your practical knowledge and understanding.
You could be asked to:
- write or identify a hypothesis or prediction that could be tested in an experiment
- name equipment and measuring instruments, and describe what they do and how they are used
- identify factors that must be controlled, and explain why
- describe how to work safely
- process data by doing calculations and representing it graphically
- identify patterns and trends in data
- evaluate the accuracy, repeatability and reproducibility of data, and identify possible errors and anomalous results
- suggest improvements to methods
- interpret data and draw conclusions from it
Practical questions will appear throughout both exam papers (Breadth and Depth), and at both Foundation Tier and Higher Tier. Practical questions could be multiple-choice or free-response, and could include calculations. A six-mark question could be asked in a practical context, so practice writing in detail about the practical work you have done.
It's important to look back at your lab book or your notes from the practical activities you have done when revising for your exams.
Sample question 1 - Foundation
Question
A group of students are conducting an experiment on Daphnia (water fleas) to investigate the effect of temperature on living things.
Daphnia are very small organisms. The students viewed the Daphnia using a light microscope.
It is possible to observe the heart of the Daphnia beating while observing it using a microscope.
The group place the Daphnia in water at different temperatures to see the effect on the heart rate of Daphnia.
Their results are shown below.
| Temperature (°C) | Group A | Group B | Group C | Group D | Mean |
| 17 | 25 | 22 | 25 | 24 | 24 |
| 20 | 27 | 27 | 25 | 25 | 26 |
| 23 | 30 | 30 | 30 | 34 | 31 |
| 25 | 33 | 57 | 36 | 39 | 36 |
| Temperature (°C) | 17 |
|---|---|
| Group A | 25 |
| Group B | 22 |
| Group C | 25 |
| Group D | 24 |
| Mean | 24 |
| Temperature (°C) | 20 |
|---|---|
| Group A | 27 |
| Group B | 27 |
| Group C | 25 |
| Group D | 25 |
| Mean | 26 |
| Temperature (°C) | 23 |
|---|---|
| Group A | 30 |
| Group B | 30 |
| Group C | 30 |
| Group D | 34 |
| Mean | 31 |
| Temperature (°C) | 25 |
|---|---|
| Group A | 33 |
| Group B | 57 |
| Group C | 36 |
| Group D | 39 |
| Mean | 36 |
What conclusion can the students draw about their experiment? [1 mark]
OCR 21st Century, GCE Biology, Paper J257, 2016.
As the temperature increases the heart rate of the Daphnia increases.
When writing a conclusion always link together the titles in the headings of the columns.
Sample question 2 - Foundation
Question
Limpets are molluscs that are found on rocky shores.
A student wants to sample a rocky shore to work out if the population of limpets differs on different parts of the shore.
Describe a method that the student could use to determine where on the rocky shore there are more limpets. [3 marks]
OCR 21st Century, GCE Biology, Paper J257, 2016.
Three from:
- Use a line transect AND quadrat. [1 mark]
- Sample along from the sea up the shore. [1 mark]
- Take many samples. [1 mark]
- Repeat at different parts of the shore. [1 mark]
Sample question 3 - Higher
Question
Read the following paragraph about classification.
"Scientists know that DNA codes for amino acids. They also know that amino acids are joined together to make proteins. By examining the sequence of amino acids in the same proteins in different animals, scientists can work out how closely related the animals are. The more similar the sequence, the more closely related organisms are. This technique is now being used to classsify organims in a completely new and more reliable way."
The table shows the sequences for eleven amino acids in humans and four other organisms.
| Organism | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | Number of differences |
| Human | Gly | Asp | Val | Glu | Lys | Gly | Lys | Lys | Ile | Phe | Ile | - |
| A | Gly | Asp | Ile | Glu | Lys | Gly | Lys | Lys | Val | Phe | Val | 3 |
| B | Gly | Asp | Ile | Glu | Lys | Gly | Lys | Lys | Ile | Phe | Val | 1 |
| C | Gly | Asp | Ile | Glu | Lys | Gly | Lys | Lys | Ile | Phe | Val | 2 |
| D | Gly | Asn | Pro | Asp | Ala | Gly | Ala | Lys | Leu | Phe | Lys | 7 |
| Organism | Human |
|---|---|
| 1 | Gly |
| 2 | Asp |
| 3 | Val |
| 4 | Glu |
| 5 | Lys |
| 6 | Gly |
| 7 | Lys |
| 8 | Lys |
| 9 | Ile |
| 10 | Phe |
| 11 | Ile |
| Number of differences | - |
| Organism | A |
|---|---|
| 1 | Gly |
| 2 | Asp |
| 3 | Ile |
| 4 | Glu |
| 5 | Lys |
| 6 | Gly |
| 7 | Lys |
| 8 | Lys |
| 9 | Val |
| 10 | Phe |
| 11 | Val |
| Number of differences | 3 |
| Organism | B |
|---|---|
| 1 | Gly |
| 2 | Asp |
| 3 | Ile |
| 4 | Glu |
| 5 | Lys |
| 6 | Gly |
| 7 | Lys |
| 8 | Lys |
| 9 | Ile |
| 10 | Phe |
| 11 | Val |
| Number of differences | 1 |
| Organism | C |
|---|---|
| 1 | Gly |
| 2 | Asp |
| 3 | Ile |
| 4 | Glu |
| 5 | Lys |
| 6 | Gly |
| 7 | Lys |
| 8 | Lys |
| 9 | Ile |
| 10 | Phe |
| 11 | Val |
| Number of differences | 2 |
| Organism | D |
|---|---|
| 1 | Gly |
| 2 | Asn |
| 3 | Pro |
| 4 | Asp |
| 5 | Ala |
| 6 | Gly |
| 7 | Ala |
| 8 | Lys |
| 9 | Leu |
| 10 | Phe |
| 11 | Lys |
| Number of differences | 7 |
Look at organisms A, B, C and D. The text in bold shows where the sequences of amino acids differ from those found in humans.
The column on the right shows the total number of these differences.
Describe and explain the conclusions that can be made from the data in the table. [3 marks]
Use the information in the paragraph above to help you.
OCR 21st Century, GCE Biology, Paper J257 - Higher, 2016.
Could include:
- B is most similar to humans because it has the fewest differences. [1 mark]
- D is most different to humans because it has the most differences. [1 mark]
- The more closely related two organisms are, the fewer the number of differences between their proteins. [1 mark]